McElroy W, Cabello E, Pillai SD; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1999 May 30-Jun 3; 99: 548 (abstract no. Q-79).
Texas A&M Research Centre, El Paso.
The availability of rapid and efficient methods to recover C. parvum oocysts from sediments and water samples is of paramount importance considering the public health implications of these organisms in environmental samples. The presence of sediments in surface and groundwater samples can impair the recovery of oocysts. Laboratory studies were conducted to determine the efficacy of the commercially available immunemagnetic system by Dynal to recover C. parvum oocysts from soils with varying textures. The rationale behind this objective was that source water concentrates can contain appreciable amounts of sediment meterial which has the potential to impair recovery efficiences may be saturated with large amounts of particulate matter and debris that may interfere with conventional oocyst recovery techniques. Soil pellets with three different textures of sand, silt and clay (soil type A= 16%, 45%,40%; soil type B= 60%, 20%,21%, soil type C= 16%, 31%, 52%) were evaluated. Each soil type were spiked with known numbers of oocysts and the oocyst recovery using a partially modified percoll-sucrose gradient centrifugation along with the immunomagnetic separation system was evaluated. Our results indicate that the sample with almost equal amounts of silt and clay had the hightest oocyst recovery (100%), while the samples with the highest sand content had only a 34% recovery These results indicated that the Dynal IMS system can have variable recovery efficiencies depending on the soil textures, however, the results also indicate that this commercially available system has value in recovering oocysts from sediments. The ability to accurately enumerate oocysts in sediments has value in environmental monitoring studies.
Publication Types:
Keywords:
- Animals
- Cryptosporidium
- Cryptosporidium parvum
- Environmental Monitoring
- Immunomagnetic Separation
- Oocysts
- Silicon Dioxide
- Soil
- Water
- Water Microbiology
- Water Pollution
- Water Purification
- Water Supply
Other ID:
UI: 102195735
From Meeting Abstracts