Marshall R, Toerner J, Kotler D, Flanigan T; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 384 (abstract no. 2120).
Case Western Reserve University, Department of Medicine, Cleveland, Ohio, USA
In persons with AIDS, Cryptosporidium parvum (Cp) infection causes life-threatening, continuous diarrhea. In contrast, immunocompetent individuals are able to mount an effective immune response and clear the infection in 2-6 weeks. Experimental infection in mice and clinical data in patients suggest mucosal and serum antibody may be important in eradicating Cp. We identified 5 HIV positive individuals with Cp infection (defined as diarrhea and Cp on two stool exams) who subsequently were able to clear the infection (normal bowel movements for 6 months and negative stool exam). In order to investigate those antigens which may be responsible for stimulating a protective immune response, sera and saliva from these individuals were analyzed by Western blot against an oocyst/sporozoite antigen preparation separated by SDS PAGE. In 3 of 5 individuals, serum antibody reacted most strongly with an approximately 80 Kd antigen. In addition, 3 of 5 sera reacted with a high m.w. antigen of approximately 130 Kd and an antigen of approximately 50 Kd. Two of 5 sera recognized a previously reported band of 23 Kd. In comparison, serum from an HIV positive patient with uncontrolled cryptosporidiosis recognized over 20 antigens from m.w. 200 to 23 Kd. In order to investigate the secretory immune response, saliva from 2 individuals who recovered from infection was analyzed by Western blot and recognized antigens of a high m.w. of approximately 130, and approximately 140 Kd. These specific antigens recognized by immune sera and saliva of HIV positive individuals who cleared infection may be important in further investigation of the immune response.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Animals
- Antibodies
- Antigens
- Antigens, Protozoan
- Blotting, Western
- Cryptosporidiosis
- Cryptosporidium parvum
- Diarrhea
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- Humans
- Immune Sera
- Mice
- Saliva
- immunology
Other ID:
UI: 102197127
From Meeting Abstracts