Stone J, Elices M, Canfield D, Dandekar S; Symposium on Nonhuman Primate Models for AIDS.
J Med Primatol. 1992 Sep-Oct; 21: abstract no. 70.
Dept. of Internal Medicine, University of California, Davis 95616.
SIV-infected rhesus macaques experiencing malabsorption had lymphocytic and histiocytic inflammatory infiltrates accompanied by high levels of SIV RNA in the jejunum. Leukocyte adhesion molecules participate in many leukocyte functions needed for immune and inflammatory responses, by specific receptor-ligand interactions. The goal of this study was to determine the expression of cell adhesion molecules prospectively in jejunum of SIV-infected animals in a time course study. Eight rhesus macaques were inoculated with SIVmac and two animals in each group were sacrificed at 2, 8, 13, and 23 weeks post-inoculation (pi). Jejunal tissues were examined for the presence of SIV infected cells and for the expression of the following cell adhesion molecules: VCAM-1, integrin beta 1, VLA-alpha 4, VLA-alpha 5, LFA-alpha 1, and MAC-alpha 1. Jejunum tissue from 7 noninfected animals served as negative controls. VCAM-1 showed essentially the same tissue distribution and intensity of staining in all animals. Smooth muscle, including the muscularis mucosa, smooth muscle within the villus core, and muscularis externa were VCAM-1+. Endothelial cells of lacteals, capillaries in the lamina propria and blood vessels within the submucosa stained positive for VCAM-1. The majority of leukocytes within lymphoid follicles were VCAM-1+. Epithelium was VCAM-1-. Integrin beta 1 stained all cells in the jejunum, including muscle, presumably due to the widespread expression of the VLA heterodimers on different cell types in the gut. The majority of mononuclear cells in the lamina propria and lymphoid follicles were VLA-alpha 4+ in all sections. Occasional intraepithelial lymphocytes were VLA-alpha 4+. VLA-alpha 5 stained smooth muscle within the villus core, muscularis mucosa, muscularis externa and endothelial cells. Lamina propria lymphocyte VLA-alpha 5 expression was decreased on all sections compared to VLA-alpha 4. Occasional intraepithelial lymphocyte staining with VLA-alpha 5 was noted. No significant difference between staining pattern was observed between infected and uninfected tissue for VLA-alpha 4 or VLA-alpha 5. Essentially all lamina propria lymphocytes and lymphocytes within lymphoid follicles were strongly LFA-1+. No significant differences between staining pattern of infected animals and noninfected animals were noted. In contrast to LFA-1, there appears to be a decrease in the percentage of MAC-1 expression on mononuclear cells within the lamina propria in one 23 week pi animal that did not produce neutralizing antibody to SIV.
Publication Types:
Keywords:
- Animals
- Cell Adhesion Molecules
- Epithelium
- Integrin alpha4beta1
- Integrin alpha5beta1
- Integrins
- Intestinal Mucosa
- Intestine, Small
- Jejunum
- Leukocytes
- Lymphocyte Function-Associated Antigen-1
- Lymphocytes
- Macaca mulatta
- Macrophage-1 Antigen
- Muscle, Smooth
- Simian immunodeficiency virus
- Vascular Cell Adhesion Molecule-1
- immunology
Other ID:
UI: 102202406
From Meeting Abstracts