Ennen J, Findeklee H, Werner K, Norley SG, Ernst M, Kurth R; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 25 (abstract no. WS-A09-5).
Paul-Ehrlich Institut, Langen, Germany.
The non-human primate African green monkey (AGM) is the natural host of a lentivirus, the simian immunodeficiency virus SIVagm. Following natural and experimental infection of AGM with SIVagm there is no evidence for the development of an immunodeficiency, in contrast to the pathogenesis in humans infected with human immunodeficiency virus type 1 (HIV-1) and macaques infected with SIVmac. In this study, we investigated the in vitro effects of CD8 T-lymphocytes on SIVagm replication in CD4 T-lymphocytes of AGM. The following results were obtained: First, in both seronegative and seropositive AGM only a very low proportion (i.e., 10%) of the peripheral blood mononuclear cells stained positive for CD4, whereas a high proportion of CD8-positive T-lymphocytes was detected (i.e, 85%). Even after persistent infection with SIVagm CD4 T-lymphocytes do not decrease in number. Second, the target cell of infection in the peripheral blood is the CD4-positive mononuclear cell and SIVagm infects cells by binding to the CD4 molecule. Third, virus isolation and virus replication of SIVagm in CD4 T-lymphocytes from seropositive AGMs is suppressed in the presence of autologous CD8 T-lymphocytes. Suppression is mediated by a soluble factor, as cell-to-cell contact is not necessary for the inhibition of virus spread in culture. Taken together, one possible reason for the apathogenicity of the SIVagm infection in AGM may be the suppression of virus replication by a soluble, yet unidentified factor secreted by CD8 T-lymphocytes quantitatively dominating among peripheral cells.
Publication Types:
Keywords:
- Animals
- Antigens, CD4
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Cercopithecus
- Cercopithecus aethiops
- DNA Replication
- HIV-1
- Humans
- In Vitro
- Simian immunodeficiency virus
- Virus Replication
- genetics
- immunology
- virology
Other ID:
UI: 102203540
From Meeting Abstracts