Goto T, Takano K, Kimura S; International Conference on AIDS.
Int Conf AIDS. 1994 Aug 7-12; 10: 166 (abstract no. PB0090).
Shionogi Biomed. Lab., Univ. of Tokyo, Japan.
OBJECTIVE: To find out good surrogate markers for analysis of pathophysiology and prediction of prognosis of HIV disease. SUBJECTS AND METHODS: Absolute counts of lymphocyte subpopulations (CD4 x CD45RA, CD4 x CD29, CD8 x CD11b, CD16 x CD56) were analyzed by two-color flowcytometry using Cytron-Absolute (Ortho) in 74 patients with HIV infection (CDC category A, B, C). In 8 patients, time courses of absolute counts of subpopulations were evaluated. Half of the patients were hemophiliacs. RESULTS: 1) CD4 counts measured by Cytron-Absolute seemed to be more reliable than those obtained through conventional method by calculation as WBC x lymphocyte (%) x CD4 (%). 2) Absolute counts of all of CD4+ subpopulations (CD4+CD45RA+, CD4+CD45RA-, CD4+CD29+, CD4+CD29-) declined almost parallel during the course of the desease. 3) Absolute counts of CD8+CD11b- (Tc) declined more slowly than CD4+ counts did. Opportunistic infections (OIs) frequently observed when CD8+CD11b- counts declined to a level below 200 cells/microliters. Thus, CD8+CD11b- counts may be a better surrogate marker for probability of OIs than CD4+ counts. 4) Absolute counts of CD16+CD56- NK cells stayed relatively constant when compared with CD16+CD56+ NK cells which declined steadily with time. CONCLUSIONS: Preliminary study on absolute counts of lymphocyte subpopulations was carried out. Some of the subpopulations seemed to be useful for the analyses of pathophysiology and prognosis of HIV disease.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Antigens, CD4
- Antigens, CD45
- Antigens, CD56
- Antigens, CD8
- CD4 Lymphocyte Count
- Color
- HIV Infections
- HIV Seropositivity
- Hemophilia A
- Humans
- Killer Cells, Natural
- Lymphocyte Subsets
- Lymphocytes
- immunology
Other ID:
UI: 102208357
From Meeting Abstracts