Cherniak R, O'Neill E, Sheng S; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1998 May 17-21; 98: 265 (abstract no. F-74).
Georgia State University, Atlanta, USA.
Cryptococcus neoformans NIH 409 was cultured in a defined medium containing one of the following as the sole carbon source: D-Xylose-1-13C, D-Mannose-1-13C, and D-Mannitol-1-13C. Glucuronoxylomannan (GXM) was isolated from the culture supernate by selected preciptitation with hexadecylmethylammonium bromide, purified by preciptitation with ethanol, and recovered by lyophilization. The GXM was treated by ultrasonic irradiation dialyzed, and recovered by lyophilization. A portion of each GXM was de-O-acetylated at pH 11.5. Each GXM and de-O-acetylated GXM was dissolved in D20 and analyzed by 1D 13C NMR spectroscopy to determine the distribution of 13C in the mannose, xylose, glucuronic acid and O-acetyl constituents. The carbon chain of Man was incorporated intact into GXM since 13C was observed only in carbon 1 of Man, GlcA, and Xyl. The carbon chain of mannitol was incorporated intact into GXM since 13C was observed only in carbons 1 and 6. This was expected since mannitol has an axis of symmetry. The carbon chain of Xyl was incorporated only in carbons 1 and 3 of Man, GlcA and Xyl. This pattern of labeling is consistent with the assimilation of Xyl through the pentophosphate pathway (hexose monophosphate shunt).
Publication Types:
Keywords:
- Acculturation
- Antigens, Fungal
- Carbon
- Carbon Isotopes
- Cryptococcosis
- Cryptococcus neoformans
- Glucuronic Acid
- Humans
- Magnetic Resonance Spectroscopy
- Male
- Mannitol
- Mannose
- Polysaccharides
- Xylose
- glucuronoxylomannan
- immunology
Other ID:
UI: 102226531
From Meeting Abstracts