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Evaluation of 9 Routine Identification Methods for Streptococcus pneumoniae.

MOUTON JW, DERKS RJ, NEELEMAN C, MEIJERS SM, KLAASSEN C; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. D-1690.

Canisius Wilhelmina Hospital, Nijmegen, Netherlands.

BACKGROUND: Several recent studies indicate that identification methods for S. pneumoniae (Sp) in the routine laboratory are suboptimal. Since the introduction of molecular techniques a true golden standard of Sp identification is available, but no large scale studies have compared routine methods with each other based here-on. METHODS: Observer blinded identification of 164 Sp and 68 non Sp was performed using: colony morphology (CP), bile solubility plate-method (BP) and tube-method (BT), sort suspension (SS), pneumoslide (PS), susceptibility to optochine using the star-method (OpCS) and quart-method (O[2] (OpCQ) and CO[2] (OpCO)) with conventional cut-off, API32 (Biomerieux; A32). The methods of reference were both a real-time fluorescence PCR with a probe constructed from the lytA gene and 16S ribotyping. Discriminant analysis (SAS software) was performed to reassess an optimal cut-off for the 3 optochine assays. RESULTS: LytA gene and 16S ribotyping were concordant. Sensitivity and specificity (%) were: CM 99, 71; BP 99, 96; BT 85, 96; SS 98, 89; PS 99,95; OpCS 96,84; OpCQ 97, 87; OpOQ 98, 72; A32 54, 100. Discriminant analysis for optochine assays did not increase the performance significantly. CONCLUSION: The bile plate methode had the best performance with a sensitivity of 99% and specificity of 96%. Optochine is nor sensitive, nor specific enough for routine identification of pneumococci and may explain discrepant Sp identifications and susceptibility results found in surveys.

Publication Types:
  • Meeting Abstracts
Keywords:
  • Antigens, Bacterial
  • Bacterial Capsules
  • Bacteriological Techniques
  • DNA Primers
  • Polymerase Chain Reaction
  • Polysaccharides, Bacterial
  • Quinine
  • Ribotyping
  • Sensitivity and Specificity
  • Streptococcus pneumoniae
  • ethylhydrocupreine
  • immunology
  • methods
Other ID:
  • GWAIDS0025513
UI: 102265137

From Meeting Abstracts




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