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Contribution of beta-Lactamase and PBP Mutations to Amoxicillin/Clavulanate Resistance in beta-Lactamase Positive Amoxicillin/Clavulanate Resistant (BLPACR) Haemophilus influenzae.

MATIC V, BOZDOGAN B, JACOBS MR, APPELBAUM PC, UBUKATA K; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. C1-682.

Hershey Medical Center, Hershey, PA.

BACKGROUND: b lactamase positive amoxicillin/clavulanate resistant (BLPACR) H. influenzae strains are very rare worldwide. Objective: Among 6,382 H. influenzae strains collected for Alexander Project (1997-2000), two strains isolated in Japan were resistant to amoxicillin/clavulanate (Amox/clav) and positive for b lactamase. This study investigated the role of b lactamase and alterations of penicillin binding proteins in resistance to Amox/clav. Method: Genes coding for b lactamases and PBP3, ftsI, were amplified by PCR and sequenced. Alterations in PBP3 were tested in 2 BLPACR and 7 BLNAR strains. H. influenzae Rd strain was used for transformation studies. RESULTS: The two BLPACR strains had TEM type b lactamase. Analysis of sequences showed 100% homology with bla TEM1. Multiple mutations were detected in ftsI gene which caused alteration in the deducted amino acid sequence of PBP3 (Table [table: see text]). Transformation of H. influenzae Rd strain with amplified ftsI gene from 2 BLPACR and 2 BLNAR strains allowed selection of Amox/clav resistant transformants that presented the same mutations as their parent strains. CONCLUSION: We concluded that in two BLPACR strains as well as BLNAR strains amoxicillin/clavulanate resistance was due to changes in PBP3. The possibility of presence of an extended spectrum beta-lactamase was excluded in BLPACR strains studied.

Publication Types:
  • Meeting Abstracts
Keywords:
  • Amoxicillin
  • Amoxicillin-Potassium Clavulanate Combination
  • Chemokines
  • Clavulanic Acid
  • Genes, Regulator
  • Haemophilus Infections
  • Haemophilus influenzae
  • Japan
  • Mutation
  • PPBP protein, human
  • Penicillin-Binding Proteins
  • Polymerase Chain Reaction
  • beta-Lactamases
  • genetics
Other ID:
  • GWAIDS0026752
UI: 102266376

From Meeting Abstracts




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