PARASHAR UD, LI J, CAMA R, MONROE S, DEZALIA M, FIGUEROA D, JIMINEZ J, GILMAN R, GLASS R; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. G-442.
CDC, Atlanta, GA.
BACKGROUND: Human caliciviruses (HuCVs) are recognized as a leading cause of outbreaks of gastroenteritis, but few studies have evaluated their etiologic role in sporadic childhood gastroenteritis. METHODS: We examined evidence of HuCV infection in sera and fecal specimens from 233 children <5 years of age seeking treatment for severe gastroenteritis at a hospital (case-patients) in Lima, Peru and in fecal specimens from 248 age- and season-matched controls. Acute- and convalescent-phase sera were tested by enzyme immunoassays (EIA) using a total of 7 baculovirus-expressed HuCV capsid antigens to look for four-fold rise in antibody titer. Fecal specimens were tested by reverse transcription-polymerase chain reaction (RT-PCR) using 2 primer sets and subsequent liquid hybridization (LH) testing of RT-PCR products using 12 genetic probes. RESULTS: A total of 128 (55%) of the 233 case-patients demonstrated evidence of HuCV infection by either EIA (N=96, 41%) or RT-PCR/LH (N=81, 35%) testing. By RT-PCR/LH testing, the detection rate of HuCVs was significantly greater among case-patients compared with controls (35% vs. 11%, p<0.001). Case-patients who seroconverted were more likely than those who did not to demonstrate HuCV infection by RT-PCR/LH testing (51% vs. 23%, p<0.001). In EIA testing, broad antigenic cross-reactivity (i.e., seroconversion with more than one HuCV antigen) was observed across genogroups and clusters, but in general seroconversion was more frequent with homologous HuCV antigens. CONCLUSIONS: HuCV infections are common and can be associated with severe gastroenteritis among Peruvian children. A collection of diagnostic assays is required to comprehensively assess evidence of HuCV infection in clinical specimens. HuCVs may rank second to rotavirus as leading viral causes of severe childhood diarrhea, underscoring the need for effective prevention and control strategies.
Publication Types:
Keywords:
- Animals
- Caliciviridae Infections
- Capsid
- Case-Control Studies
- Child
- Child Day Care Centers
- DNA Primers
- Disease Outbreaks
- Feces
- Gastritis
- Gastroenteritis
- Humans
- Immunoenzyme Techniques
- Inflammatory Bowel Diseases
- Norwalk virus
- Peru
- Reverse Transcriptase Polymerase Chain Reaction
- Rotavirus
- Rotavirus Infections
- Rotavirus Vaccines
- Virus Diseases
Other ID:
UI: 102268029
From Meeting Abstracts