Carnahan A, Ali A, Kriss J, Daugherty S, Hayat A, Johnson RJ, Johnson JA; Interscience Conference on Antimicrobial Agents and Chemotherapy.

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1996 Sep 15-18; 79 (abstract no. D108).

University of Maryland, School of Medicine, Baltimore, MD.

Seventy-three isolates of clinically significant Vibrios representing 11 species were conventionally identified. These included 25 Vibrio cholerae including serogroup O139 (n=5), 01 (n=10) and other serogroups (n=10); V. parahaemolyticus (n=10); V. vulnificus (n=10); others (n=28). Each isolate was then identified using BELO Crystal Enteric/Nonfermenter ID system (Becton Dickinson), API 20E and Vitek GNI card (Biomeriux Vitek, Inc.). 56.2% (41/73, 71.2% (52/73) and 74.0% (54/73 were correctly identified by API 2OE, Crystal ID, and Vitek respectively. 12.3% (9/73), 10.9% (8/73), and 17.8% (13/73) were incorrectly identified by API 20E, Crystal ID, and Vitek. API 20E, Crystal ID and Vitek did not give an identifcation, and suggested additional testing, for 31.5% (23/73), 17.8% (13/73), and 1.4% (1/73) of isolates respectively. Both API 20E and Crystal ID had tests that were routinely very difficult to interpret. Every system performed acceptably for identification of all serogroups of V. cholerae tested including the emerging O139 Bengal strains. However, further tests should be done to confirm identification of other medically important Vibrios including V. parahaemolyticus and V. vulnificus.

Publication Types:

• Meeting Abstracts

Keywords:

• Evaluation Studies
• Vibrio
• Vibrio Infections
• Vibrio cholerae
• methods

Other ID:

• 98927631

UI: 102234805

From Meeting Abstracts