OLSZYNA DP, OPAL SM, PRINS JM, HORN DL, SPEELMAN P, VAN DEVENTER SJ, VAN DER POLL T; Interscience Conference on Antimicrobial Agents and Chemotherapy.

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1999 Sep 26-29; 39: 388 (abstract no. 1611).

Academic Med. Ctr., Amsterdam, NETHERLANDS

BACKGROUND: Chemokines are a superfamily of chemotactic proteins. CXC chemokines act primarily on granulocytes, cells often found in urine of patients with urinary tract infection (UTI). Aim andMETHODS: To obtain insight into systemic and local production of CXC chemokines during UTI, epithelial cell-derived neutrophil-activating protein (ENA)-78, growth-related oncogene (GRO)alpha and interleukin(IL)-8 were measured in plasma and urine of 20 healthy individuals, 33 patients with urosepsis and in 11 healthy humans after iv injection of endotoxin (LPS lot G, 4 ng/kg). Statistics by Mann-Whitney U test and ANOVA.RESULTS: Chemokine levels were low or undetectable in plasma and urine of healthy controls. In patients with urosepsis, ENA-78, GROalpha and IL-8 were found at increased concentrations in plasma (0.12 (<0.03-6.57), 0.21 (0.07-2.05), 0.03 (0.01-0.65) ng/ml, resp.); (median (range)), (all P<0.05 vs controls) and urine (0.27 (<0.03-1.66), 0.10 (<0.03-0.51), 0.38 (<0.01-3.13) ng/ml, resp.), (all P<0.001). LPS given iv to healthy individuals without UTI induced increases in plasma levels of all three chemokines. ENA-78 peaked at 4 h (0.45 +/- 0.52 ng/ ml; mean +/- SE); GROalpha at 3 h (0.47 +/- 0.51 ng/ml) and IL-8 at 3 h (1.79 +/- 0.16 ng/ml), (all P<0.005). After iv LPS, urine ENA-78 and GROalpha remained low, whereas IL-8 levels increased to 0.01 (<0.01-0.03) ng/ml (P<0.005 vs prior to LPS) between 3 and 6 h post-LPS. Urine/Plasma ratios of all chemokines were significantly higher in patients with urosepsis than in healthy humans exposed to LPS.CONCLUSION: In patients with urosepsis urine, but not plasma, levels of ENA-78, GROalpha and IL-8 are higher than in healthy subjects injected with LPS. These data suggest that CXC chemokines are produced locally within the urinary tract during UTI.

Publication Types:

• Meeting Abstracts

Keywords:

• CXCL5 protein, human
• Chemokines
• Chemokines, CXC
• Endotoxins
• Humans
• Interleukin-8
• Lipopolysaccharides
• Neutrophils
• Plasma
• Sepsis
• Urinary Tract Infections
• immunology
• urine

Other ID:

• GWAIDS0007961

UI: 102245458

From Meeting Abstracts