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Identification of Klebsiella Species Based on Signature Nucleotide Patterns within a 363 Nucleotide Long Sequence of the 16S rRNA Gene.

HANSEN DS, BOYE K; Interscience Conference on Antimicrobial Agents and Chemotherapy.

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2000 Sep 17-20; 40: 150.

Statens Serum Institut, Copenhagen, Denmark

BACKGROUND: The prevalence of K. terrigena and K. planticola in clinical specimens is poorly known since these species are misidentified as K. pneumoniae and/or K. oxytoca in the routine clinical microbiology laboratory. We have identified signature nucleotide patterns (SNP) for each Klebsiella species within a short 16S rRNA gene sequence. Using these SNP on biochemically well characterized strains we have evaluated this approach for easier characterization of Klebsiella species from clinical specimens.METHODS: 157 Klebsiella strains identified by 38 specialized biochemical tests as typical representatives of their respective species were included. A 363 bp sequence of the 16S rRNA gene was amplified by PCR and sequenced. The Klebsiella species were identified by their SNP.RESULTS: All strains fitted one of the Klebsiella SNP. Correlation between SNP and biochemical identification was: K. pneumoniae 47/47, K. oxytoca 26/26, K. planticola 8/30, K. terrigena 25/28, K. ornithinolytica 16/16, and Enterobacter aerogenes (K. mobilis) 10/10. The biochemically identified K. planticola (n=22) and K. terrigena (n=3) strains were by SNP identified as K. ornithinolytica and K. planticola, respectively. Conclusion: All K. pneumoniae and K. oxytoca strains could be identified by their SNP. Divergence between SNP and biochemical identification was only found within the ``new environmental Klebsiella species. The 22 divergent K. planticola strains (K. ornithinolytica SNP) reflect the unsolved taxonomy for these two species (some report that ODC positive Klebsiella strains is a distinct biogroup of K. planticola). In conclusion, identification of Klebsiella to species level by SNP is a promising method for easier characterization of Klebsiella strains from clinical specimens.KEYWORDS: Identification; Klebsiella; RRNA

Publication Types:
  • Meeting Abstracts
Keywords:
  • Base Sequence
  • Enterobacter aerogenes
  • Klebsiella
  • Klebsiella Infections
  • Klebsiella pneumoniae
  • Polymerase Chain Reaction
  • classification
  • genetics
Other ID:
  • GWAIDS0010890
UI: 102248388

From Meeting Abstracts




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