STEPHAN B, PRIDMORE A, SILLEY P; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. F-420.

Bayer AG, Leverkusen, Germany.

BACKGROUND: Pradofloxacin (PRA), a novel 8-cyano fluoroquinolone, is being developed for the treatment of bacterial infections in dogs and cats. In addition to the Gram-negative spectrum it shows enhanced activity against Gram-positive bacteria. This study determined the in vitro activity of PRA and metronidazole (MTR) against veterinary anaerobes. METHODS: A total of 178 obligate anaerobes were isolated from canine and feline clinical cases (wound infections and dental abscesses) and healthy carriers (faecal samples and oral swabs). Predominant genera were Clostridium (n = 39), Bacteroides (n = 37), Prevotella (n = 25) and Fusobacterium (n = 24). The MIC of each antimicrobial was determined by agar dilution methodology (NCCLS document M11-A5, 2001) in a GLP-compliant study, which included Bacteroides fragilis ATCC 25285 and Eubacterium lentum ATCC 43055 as quality control strains. Results were expressed as MIC[50], MIC[90] and geometric mean MIC (GMIC) in [microg/ml]. RESULTS: Antibacterial activities of PRA and MTR are compared in the table below: [table: see text] For the total panel of 178 anaerobes, the MIC range was 0.016-16 for PRA and 0.031-256 for MTR. The MIC[50] values of PRA/MTR were 0.5/0.5 for Clostridium, 0.25/0.5 for Bacteroides, 0.125/0.5 for Prevotella and 0.5/0.25 for Fusobacterium. The MIC[50] of PRA against oral Porphyromonas strains (n = 8) was 0.062, the GMIC 0.09. The comparative values for MTR were 0.125 and 0.25. CONCLUSIONS: Pradofloxacin and metronidazole exhibited comparable in vitro activity against anaerobic bacteria with a tendency for pradofloxacin to be more active.

Publication Types:

• Meeting Abstracts

Keywords:

• Animals
• Anti-Bacterial Agents
• Anti-Infective Agents
• Bacteria, Anaerobic
• Bacterial Infections
• Canidae
• Cats
• Dogs
• Fluoroquinolones
• Gram-Positive Bacteria
• In Vitro
• Metronidazole
• Microbial Sensitivity Tests

Other ID:

• GWAIDS0026055

UI: 102265679

From Meeting Abstracts