MOOIJ M, SCHOUTEN I, SCHULTSZ C, SAVELKOUL P, REINDERS E, VOS M, VAN BELKUM A, VANDENBROUCKE-GRAULS CM; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. C2-1993.

VU University Medical Center, Amsterdam, Netherlands.

BACKGROUND: In hematology wards from 2 hospitals, a rapid increase in the isolation rate of ciprofloxacin-resistant E.coli (crEco) was observed. Selective decontamination of the gut regimens on these wards consisted of ciprofloxacin or colistin with tobramycin. To study whether the increase was due to clonal spread or was integron associated, isolates were characterized by AFLP and with respect to the presence and type of integrons. METHODS: 59 crEco and 81 sensitive isolates from both hematology wards and 23 crEco obtained from other wards, were characterized by AFLP, integrase- and integron PCR and by RFLP of integrons. Data were combined and analysed using Bionumerics. RESULTS: AFLP indicated that the strains were heterogeneous. 65 (80%) of crEco contained the integrase I gene, compared to 8 (10%) of the sensitive strains (p<0.001). 10 (12%) of crEco contained the integrase II gene compared to 12 (15%) of the sensitive strains. PCR-RFLP analysis of the integron I cassettes revealed the presence of 4 patterns. Type A was the most common integron type (29/62,47%), irrespective of the hospital or ward on which the strains were isolated. 88% of crEco were resistant to cotrimoxazole compared to 10% of sensitive strains. Conclusion: Within 163 E.coli strains from two different hospitals, which were heterogeneous as determined by AFLP, 4 distinct PCR-RFLP types of integron I cassettes were present. Ciprofloxacin resistance was associated with the presence of integron type I although such resistance has not been reported to be integron mediated, in contrast to resistance to cotrimoxazole. The possible association between ciprofloxacin use and integron I mediated resistance warrant further studies.

Publication Types:

• Meeting Abstracts

Keywords:

• Ciprofloxacin
• Drug Resistance
• Drug Resistance, Bacterial
• Drug Resistance, Microbial
• Drug Resistance, Multiple, Bacterial
• Escherichia coli
• Escherichia coli Infections
• Integrases
• Integrons
• Polymerase Chain Reaction

Other ID:

• GWAIDS0027744

UI: 102267368

From Meeting Abstracts