Lacroix M, Chalifour R, Stern MD, Dionne G; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 370 (abstract no. W.B.P.110).
IAF BioChem International Inc., Laval, Quebec, Canada, H7V 1B7
OBJECTIVE: Report on the superiority of cyclic peptides of the transmembrane glycoproteins over their linear counterparts as antigens in an ELISA for the highly sensitive detection of antibodies to HIV-1 and to HIV-2. METHODS: Peptides covering an immunodominant region of HIV-1 and of HIV-2 transmembrane glycoproteins were synthesized. A fraction of the purified peptides was cyclized under conditions in which the formation of dimers and polymers is minimized. Cyclic peptides were then isolated by HPLC and the absence of residual thiol function confirmed by Ellman's test. RESULTS: Serially diluted serum samples were prepared from confirmed HIV-1 and from confirmed HIV-2 seropositive patients. Each serum dilution was tested on linear and cyclic peptide-coated microtiter wells. Serum titers measured on cyclic peptide-coated wells were 1.2 to 43 times higher than those obtained on linear peptide-coated wells. CONCLUSION: We have found that cyclic peptides mimicking an immunodominant region of HIV are superior antigens than their linear counterparts and that they can form the basis of highly sensitive and specific tests for HIV-antibody detection.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Enzyme-Linked Immunosorbent Assay
- HIV Antibodies
- HIV Infections
- HIV Seropositivity
- HIV-1
- HIV-2
- Humans
- Immunodominant Epitopes
- Peptides
- Peptides, Cyclic
- immunology
Other ID:
UI: 102177744
From Meeting Abstracts