Lacroix M, Penney CL, Dwyer RJ, Stern MD, Bellini F, Dionne G, Tsoukas C; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 370 (abstract no. W.B.P.111).
IAF BioChem International Inc., Montreal, Can., H7V 1B7
Although many assays for HIV-1 antibodies are now on the market, they do not always perform as well as claimed by the manufacturers. Clearly, there is an urgent need for a more specific and more sensitive HIV antibody test. Our assay uses synthetic peptides mimicking highly conserved and immunodominant epitopes of gp41 and gp120. It is very sensitive (100%) and very specific (99.8%). Among the 1250 confirmed negatives tested were 250 samples from patients suffering diseases often associated with false-positive ELISA testing (lupus, rheumatoid arthritis, blood fraction recipients, various infectious diseases). Seropositive specimens were obtained from a wide spectrum of risk groups. Our test is more sensitive than WB and is equivalent to RIPA and IFA in detecting early seroconverters. The test also detects HIV-2 antibodies since the peptide cocktail includes one homologous to a region of the HIV-2 gp40 protein. The ELISA is done at room temperature and requires 90 min. to complete. CONCLUSION: Our kit is more reliable than others since it has the highest sensitivity and a specificity for both HIV-1 and HIV-2.
Publication Types:
Keywords:
- Enzyme-Linked Immunosorbent Assay
- HIV Antibodies
- HIV Envelope Protein gp120
- HIV-1
- HIV-2
- Humans
- Indicators and Reagents
- Peptides
- Proto-Oncogene Proteins c-kit
- Sensitivity and Specificity
- immunology
Other ID:
UI: 102177745
From Meeting Abstracts