Huang M, Hanna Z, Aziz D, Simard C, Jolicoeur P; International Conference on AIDS.

Int Conf AIDS. 1989 Jun 4-9; 5: 529 (abstract no. W.C.O.28).

Clinical Research Institute of Montreal, Montreal, Quebec, Canada

The Duplan strain of murine leukemia virus (MuLV) induces MAIDS, a disease showing striking similarities with human AIDS. We identified the etiologic agent of the disease as being a defective retrovirus having a 4.8 kbp genome. Sequencing of this DNA showed that the pol and env genes have been deleted and that the complete gag region has been conserved and harbors a novel p12 sequence. Using a cell-free translation system, the genome has been shown to encode a 60 kd gag fusion protein. The same protein was detected in non-producer cells harboring the defective genome and is not cleaved. N-tropic pseudotypes have been constructed with this virus and the role of helper virus in the disease is also being investigated using the psi2 encapsidation-negative system. This mouse model emphasizes the need to search for pathogenic defective retroviruses in AIDS.

Publication Types:

• Meeting Abstracts

Keywords:

• Animals
• Base Sequence
• DNA
• Gene Products, gag
• Genes, env
• Genes, gag
• Genes, pol
• Genome
• Helper Viruses
• Humans
• Leukemia Virus, Murine
• Mice
• Murine Acquired Immunodeficiency Syndrome
• Retroviridae
• genetics

Other ID:

• 00273389

UI: 102178635

From Meeting Abstracts