Mariotti M, Lefrere JJ, Berriche S, Vittecoq D, Noel B, Ferrer-le-Coeur F, Girot R, Rouger P; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 588 (abstract no. T.C.P.131).
F.N.T.S., Paris, France
OBJECTIVE: Use of Polymerase Chain Reaction (PCR) in human immunodeficiency virus (HIV)-infected subjects and in seronegative but high risk subjects. METHODS: Localisation on HIV 1 genome and nucleotide sequence of the primers and probes used (derived from conserved regions of the viral genome) were: TABULAR DATA, SEE ABSTRACT VOLUME. Slot-blot and Southern-blot analysis were used in detection of HIV sequences. 530 subjects were studied: 200 non-haemophiliac HIV seropositive (Elisa with confirmation by Western-blot) subjects (amongst them, ten were seronegative 6 months earlier); 40 haemophiliac patients (20 seropositive + 20 seronegative); 25 AIDS patients; 25 seronegative polytransfused thalassaemia patients; 20 subjects with isolated and persistent anti-core antibodies (anti-p25 or anti-p18); 20 seronegative partners of HIV seropositive subjects (a second sample was collected and studied 6 months later in all); 200 healthy seronegative blood donors as controls. RESULTS: 1) PCR is positive in the HIV seropositive subjects but some appear negative in this assay when using only one single primer pair and probe. The use of three sets of primers appears necessary to prevent false-negative results in this context. 2) No detection of HIV DNA was observed in seronegative subjects from high risk groups. 3) All control subjects were PCR negative. CONCLUSION: PCR allows the identification of HIV DNA in seropositive subjects and confirms the absence of HIV infection in seronegative subjects.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Base Sequence
- Blood Donors
- Blotting, Southern
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- HIV
- HIV Antibodies
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- Hemophilia A
- Humans
- Polymerase Chain Reaction
- genetics
- immunology
Other ID:
UI: 102178990
From Meeting Abstracts