Hall WW, Kaplan MH, Farber B, Coronesi M, Salahuddin SZ, Oyaizu N, Nagashima K; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 617 (abstract no. Th.C.P.5).
North Shore University Hospital, Cornell University, Manhasset, NY., USA
OBJECT: To study concomitant infection of HIV and HTLV I/II METHODS: HIV patients (pts) were screened for antibody (Aby) to HTLV I/II. Aby + sera was confirmed using Western blot technology and radioimmunoprecipitation technology (RIPA) to purified HTLV I NS-1 and HTLV II 580B. Peripheral blood lymphocytes were grown with IL-2 and PHA. After rounds of lysis, surviving cells were cocultivated with cord blood lymphocytes (CBLs) and continuous cell lines established. Viral isolation was confirmed with reverse transcriptase assay, electron microscopy and Southern hybridization technology. RESULTS: 30 sera from 383 HIV individuals had Aby to HTLV I/II mostly in older intravenous drug users (IVDU). 6 new isolates of HTLV II were made from 6 IVDUs. 3 isolates of HTLV I were made from 3 HS pts. Patients with HTLV II had ichthyosis, dermatopathic lymphadenopathy and staph infection. Those with HTLV I had Tropical spastic paraparesis and CD-8 chronic leukemia. Restriction enzyme mapping of 4 strains of HTLV II showed all 4 were similar to 2 previous isolates of HTLV II (Mo and NRA) indicating that the virus of HTLV II is highly conserved. CONCLUSION: Significant HTLV II infection is in New York IVDU's.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Blotting, Western
- Communicable Diseases
- Deltaretrovirus
- Deltaretrovirus Antigens
- Deltaretrovirus Infections
- HIV
- HIV Antibodies
- HIV Infections
- HIV Seropositivity
- HIV Seroprevalence
- HIV-1
- HTLV-I Infections
- HTLV-II Infections
- Human T-lymphotropic virus 1
- Human T-lymphotropic virus 2
- Humans
- New York
- Paraparesis, Tropical Spastic
- RNA-Directed DNA Polymerase
- Substance Abuse, Intravenous
- immunology
Other ID:
UI: 102179160
From Meeting Abstracts