Amadori A, Zamarchi R, Veronese ML, Francavilla E, Barelli A, Borri A, Chieco-Bianchi L; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 640 (abstract no. Th.C.P.144).
Institute of Oncology, University of Padova, Padova, Italy
OBJECTIVE: To better define B-cell activation during HIV-1 infection. METHODS: Limiting dilution assay of B-cell precursors spontaneously secreting HIV-1-specific and total Ig; absorption of culture supernatants with solid-phase HIV-1; effect of T-cell and monocyte removal, and interleukin addition, on in vitro spontaneous Ig synthesis. RESULTS: In vitro spontaneous Ig synthesis did not depend on T-cell presence, while monocyte removal caused a striking fall in spontaneous Ig production. IL-6 presence in culture was essential; antisera against IL-6 strongly reduced spontaneous Ig synthesis, which was partly restored by rIL-6 addition to monocyte-depleted populations. The frequency of B-cell precursors spontaneously producing HIV-1-specific Ab was about 1/3-1/4 of that of spontaneously activated, Ig-secreting precursors. Moreover, after absorption with solid-phase HIV-1, about 30% of total Ig was removed from unstimulated culture supernatants. CONCLUSIONS: HIV-1-specific B-cell activation is a major constituent of the overall B-cell activation in PBL from seropositive subjects. This phenomenon can contribute to the virus-induced immune damage, and partly explain the increased frequency of AIDS-associated B-cell malignancies.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- B-Lymphocytes
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Infections
- HIV Seropositivity
- HIV-1
- Immunoglobulins
- In Vitro
- Interleukin-6
- Interleukins
- Lymphocyte Activation
- Monocytes
- T-Lymphocytes
- immunology
Other ID:
UI: 102179298
From Meeting Abstracts