Chrystie IL, Almeida JD, Banatvala JE; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 689 (abstract no. C.784).
Department of Virology, United Medical and Dental Schools of Guys and St Thomas's, St Thomas's Campus, London, United Kingdom
Negative staining EM has proved less rewarding than thin sectioning in the study of HIV morphology and antigenic structure. This may, in part, be due to the close association of HIV with the propagating cell making it difficult to obtain good preparations of isolated virus. However, when HIV-2 infected cells (CB L-20/C8166) are gently homogenised in a Tenbroek-type, glass/teflon homogeniser surface located virus is sheared off and can be concentrated by simple differential centrifugation. The particles so obtained are mostly clumped and very little internal morphology is visible. However the greater number of particles isolated make it possible to visualize the immature virus particle which contains a spherical, doughnut shaped core. Subsequent treatment of the semi-purified virus with a mixture of detergent (Nonidet P40) and glutaraldehyde both releases and stabilises the internal cone shaped component. Concentrations of both detergent and glutaraldehyde are critical, but when conditions are correct reasonable amounts of antigenically reactive cores are available for immune EM. These HIV-2 cores react positively with their corresponding human antisera, giving rise to classical immune complexes. These complexes contain various aberrant forms of the cone shaped core ranging from a squat triangular form to elongated rods. Their presence within the aggregates establishes that, antigenically, they are similar to, if not identical with, the standard cone shaped internal component. The isolated cores also reacted positively with a mouse monoclonal antibody directed against p24 (HIV-1) which reacts also with p25 (HIV-2).
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Animals
- HIV
- HIV Antibodies
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- HIV-2
- Humans
- Immune Sera
- Mice
- Virion
- anatomy & histology
- immunology
Other ID:
UI: 102179532
From Meeting Abstracts