Maslak DM, Schmerr MJ; American Society for Microbiology. Meeting.
Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9; 91: 339 (abstract no. T-32).
National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA.
Ovine progessive pneumonia virus (OPPV) is a lentivirus that infects sheep and causes a multi-systemic disease which can lead to death. The antigenic relatedness between HIV-1 and OPPV was examined by immunoblot analysis. Fourteen out of 20 sheep sera that were positive for OPPV on an immunodiffusion test reacted with HIV-1 p24 on a commercial blot SDS-PAGE treated HIV-1 infected cell lysate (Virostat). These 14 sheep sera did not react with the negative control (blot of SDS-PAGE non-infected cell lysate). The same results were obtained when blots containing segregated recombinant HIV-1 proteins (Dupont) were used. There was no correlation between the ability to cross-react to HIV-1 proteins and the status of disease in the sheep. Polyclonal goat anit-HIV-1 (Virostat) reacted with p17 on a blot of SDS-PAGE of OPPV infected cell lysate. The polyclonal antisera did not react on a blot of SDS-PAGE of non-infected cell lysate. Although OPPV is more distantly related to HIV-1 genomically than other lentiviruses such as SIV and BIV, the serological cross-reactivity between HIV-1 and OPPV antigens is reciprocal. The cross-reactivity shown in this study probably occurs in regions that are conserved in the gag protein of the lentiviral genome.
Publication Types:
Keywords:
- Gene Products, gag
- Goats
- HIV-1
- Humans
- Immunodeficiency Virus, Bovine
- Immunodiffusion
- Lentiviruses, Ovine-Caprine
- Pneumonia, Progressive Interstitial, of Sheep
- Sheep
- Sheep, Domestic
Other ID:
UI: 102181547
From Meeting Abstracts