Sugihara K, Reichart PA, Gelderblom HR, Pohle HD, Langford A, Schmidt-Westhausen A, Reupke H; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 215 (abstract no. Th.B.375).
Kagoshima University Dental School, Kagoshima, Japan
OBJECTIVE: Purpose of the present study was to evaluate two different EM techniques suitable to detect Epstein-Barr virus (EBV) in oral hairy leukoplakia (HL). METHODS: A comparative study using the conventional two step method of negative staining (CNS) and negative staining after airfuge enrichment (ANS) was performed. For the study of CNS scrape specimens from lateral borders of tongue of 12 HIV-infected patients; for ANS scrape specimens of 54 HIV-positive patients were available. As controls served 3 patients with infectious mononucleosis and 4 HIV-negative patients. RESULTS: Using CNS, EBV particles were detected in 4 out of 16 clinically diagnosed HL cases (25%). In the second group (ANS) HL was observed clinically on 41 tongues; in 35 instances EBV was detected (85.4%). HIV negative controls were EBV negative, mononucleosis patients were positive in two instances without clinical appearance of HL. CONCLUSION: Whereas CNS of HL scrapes allowed for detection of EBV in only 25% the evaluation by ANS revealed - as expected - a high number of positive results (85.4%). It is, however, also interesting to find EBV in clinically normal oral mucosa. This finding indicates that EBV expression precedes clinical appearance of HL.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- HIV Infections
- HIV Seropositivity
- Herpesvirus 4, Human
- Humans
- Infectious Mononucleosis
- Leukoplakia, Hairy
- Mouth Mucosa
- Tongue
Other ID:
UI: 102181940
From Meeting Abstracts