Nagy K, Dobronyi I, Timar I; International Conference on AIDS.

Int Conf AIDS. 1991 Jun 16-21; 7: 92 (abstract no. M.A.1002).

Institute of Isotopes, Hungarian Academy of Sciences and Department of Pathology, Semmelweis Medical University, Budapest, Hungary

OBJECTIVE: To evaluate the role of FcRII(CD32) of CD4 negative human machrophage cells in receptor mediated enhancement of HIV infection. METHODS: Membrane markers characterisation of the machrophes cell line DD were done by immunofluorescence (IF), DD cells infected with HIV directly or complexed with HIV-specific antibodies. Viral replication were monitored by IF, reverse transcriptase (RT) assay and electron microscopy (EM). RESULTS: DD cells express CD14 antigens and FcRII (CD32) receptors onto the cell membrane. Cells are negative for FcRI, Leu M3, HLA-DR and also for CD4, even after receptor induction by vitamin D3. Dot blot hybridisation for CD4 mRNA was also negative. DD cells were not permissive for direct infection with HIV-1 (BCO41) and HTLV-3B. Treatment of cells by immunocomplexes resulted in moderate CPE but IF and RT assay were negative. EM, however, revealed HIV particles in cytoplasmic vacuoles of DD cells treated with highly diluted ( 24.000) viral: immunocomplex. CONCLUSIONS: HIV had been endocytosed in form of immunocomplex mediated by the FcRII receptors of CD4 negative DD cells. Because of the low rate viral replication, particles accumulated in cytoplasmic vacuoles and have not been expressed into culture medium. Observation suggests that similarly to FcRI, FcRII receptor mediated enhancement represents an alternative infectious pathway independent of CD4.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Antigens, CD4
• CD4-Positive T-Lymphocytes
• FCGR2A protein, human
• Fc gamma receptor IIC
• HIV
• HIV Antibodies
• HIV Core Protein p24
• HIV Envelope Protein gp120
• HIV Infections
• HIV Seropositivity
• HIV-1
• Humans
• Macrophages
• RNA-Directed DNA Polymerase
• Receptors, HIV
• Receptors, IgG
• immunology

Other ID:

• 1100291

UI: 102182565

From Meeting Abstracts