Demirhan I, Chandra A, Hofmann D, Chandra P; International Conference on AIDS.

Int Conf AIDS. 1991 Jun 16-21; 7: 107 (abstract no. M.A.1060).

Laboratory of Molecular Biology (ZBC) Frankfurt University Medical School, FRG

OBJECTIVE: Transactivation of HIV-1 LTR is the most important event in the expression of structural genes. Our aim is to look for endogenous factors which affect this process in target cells (CD4 cells and macrophages) modulating the pathophysiological state of the disease. METHODS: Jurkat cells or U937 cells were transfected with plasmids containing HIV-1 LTR-CAT or tat gene sequences (FEBS Lett. 236: 282-286, 1988). Transfected cells were cultivated with or without the test compounds. CAT assays were carried out in triplicate. RESULTS: L-Thyroxine was found to inhibit CAT expression in U937 cells to 45% at 1muM, but no inhibition was observed in Jurkat cells. Prostaglandins A1 and B1 showed a 30% inhibition in U937 cells at 5muM, but again no inhibition in Jurkat cells. Prostaglandin D2 inhibited the transactivation in U937 cells to about 45% at 5muM, but showed a stimulation to 191% in Jurkat cells. Prostaglandins E1 and E2 had a similar effect in both types of cells, showing about 25% inhibition at 5muM. Prostaglandin F1 alpha had no effect in both cell lines. CONCLUSIONS: Hypothyroxinemia has been reported in patients in a progressive state of the disease. Our results with L-Thyroxine and prostaglandins may be interesting for pathophysiological investigations.

Publication Types:

• Meeting Abstracts

Keywords:

• Animals
• Base Sequence
• CD4-Positive T-Lymphocytes
• Cats
• Cell Line
• Gene Products, tat
• Genes, tat
• HIV Long Terminal Repeat
• Humans
• Jurkat Cells
• Macrophages
• Prostaglandins
• Thyroxine
• Trans-Activation (Genetics)
• U937 Cells
• genetics

Other ID:

• 1106091

UI: 102182623

From Meeting Abstracts