Gibb AP, Van Den Elzen H; Interscience Conference on Antimicrobial Agents and Chemotherapy.

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1998 Sep 24-27; 38: 270 (abstract no. F-147).

University of Calgary, Alberta, Canada.

The MIC of Syn2869 (SynPhar Laboratories Inc. - Taiho Pharmaceuticals Co.) was determined for 139 distinct consecutive clinical isolates of yeasts from sterile sites in one tertiary-care hospital, and compared with the MICs of fluconazole and itraconazole. Yeast isolates were obtained from blood cultures and other normally-sterile clinical specimens submitted to Foothills Hospital microbiology laboratory for routine diagnostic testing. All yeast isolates obtained were tested for germ-tube production, and if negative identified by Vitek Yeast Biochemical Card, with morphological confirmation for non-candida species. Where more than one isolate of the same species was obtained from an individual patient within a 7-day period only a single representative isolate was selected for susceptibility testing, except that in three instances where both blood and CSF isolates were obtained and both were included. Broth microdilution susceptibility tests were performed by NCCLS M27-T. For Syn2869 solutions were made in DMSO and diluted as recommended for water-insoluble agents. MICs were read visually after 48 h incubation by two technologists, and also read spectrophotometrically after shaking at 492 nm on a Dynatek microplate reader. The lowest concentration well with an OD of less than 50% of the control well was considered to be the MIC by the spectrophotometric method. Where the visual and spectrophotometric MICs differed the modal or middle reading was selected. Of the 139 strains tested 83(59%) were Candida albicans, 18(13%) were C. glabrata, and 38(27%) were other species. MIC90 (ug/mL) for fluconazole, itraconazole and Syn2869 respectively were as follows; C. albicans: 0.25, < or = 0.03, 0.06; C. glabrata: > or = 64, 4, 4; other species: > or = 64, 0.5, 0.25. The high fluconazole MIC90 of the "other species" was accounted for by 6 C. paratropicalis with MICs of > or = 64. These 6 isolates had itraconazole MICs of < or = 0.5, and Syn2869 MICs of < or = 0.25. The in vitro activity of Syn2869 in this study is superior to that of fluconazole, but similar to that of itraconazole.

Publication Types:

• Meeting Abstracts

Keywords:

• Candida
• Fluconazole
• Humans
• In Vitro
• Itraconazole
• Microbial Sensitivity Tests
• Saccharomyces cerevisiae
• Yeasts

Other ID:

• 20710742

UI: 102188048

From Meeting Abstracts