Herndier B, Shiramizu B, Jewett N, Young L, Reyes G, McGrath M; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 116 (abstract no. W.A.1098).
University of California, San Francisco, San Francisco General Hospital, CA, USA
OBJECTIVE: A patient with non-Hodgkin's lymphoma is described who had a rapid deteriorating clinical course. Immunophenotyping and molecular analysis was undertaken to characterize the tumor. METHODS: Immunophenotyping was performed using appropriate controls. Representative samples were processed for Southern blot analysis. Probes: Ig, T-cell receptor, HIV-1 (3'LTR), and HTLV-I. RESULTS: The histology of the pulmonary tumor was consistent with a large cell lymphoma. The immunophenotype was of a CD4+, CD25+ T-cell tumor. B-cell markers were negative. Anti-HIV p24 monoclonal antibody immunostaining was strongly positive. Southern blot analysis revealed a rearranged C(T)beta gene. Hybridization showed HIV-1 sequences by the presence of 6kb and 3kb bands with BamHI digest in addition to a band of about 9.6kb with XbaI digest. The tumor was negative for HTLV-1. DISCUSSION AND CONCLUSION: The BamHI and XbaI digests suggests an integrated HIV with an internal BamHI site and flanking XbaI sites. This appears to be the first case of AIDS-associated lymphoma arising from an HIV-infected progenitor cell. Mechanisms by which transformation can occur include: integration near a cellular oncogene, induction of cellular oncogenes by transactivating regulatory gene products, or mutation to an oncogenic retrovirus. A clone of the integrated HIV has been isolated and further analysis will determine the form of this retrovirus.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigens, CD4
- HIV Infections
- HIV Seropositivity
- HIV-1
- HTLV-I Infections
- Human T-lymphotropic virus 1
- Humans
- Immunophenotyping
- Lymphoma, AIDS-Related
- Lymphoma, Non-Hodgkin
- Lymphoma, T-Cell
- T-Lymphocytes
- immunology
Other ID:
UI: 102192290
From Meeting Abstracts