Furlini G, Re MC, Dalla Casa P, Bianchi S, Guerra B, La Placa M; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 177 (abstract no. W.A.1340).
Institute of Microbiology, University of Bologna, Italy
OBJECTIVE: The diagnosis of HIV-1 infection in children born to seropositive mothers is hampered by the presence of passively acquired antibodies of maternal origin. The objective of the research was to investigate the significance of anti HIV-1 IgA in the diagnosis of active infection. METHODS: Seventy children born to HIV-1 seropositive mothers, followed up to 15-24 months after birth were studied for the presence of specific antibody (IgG, IgA, and IgM) and for the presence of active infection (p24 antigenemia, virus isolation). RESULTS: Eleven children were found HIV-1 infected (persistent p24 antigenemia and/or positive virus isolation). Results obtained have shown that IgA antibodies to low molecular weight HIV-1 polypeptides were persistently present in the great majority of infected babies (8/11), while IgM antibodies was found in a lower number of cases (6/11). The sequential analysis of sera from seropositive babies with no signs of HIV-1 infection did not reveal the presence of any IgA or IgM reactivity to HIV-1 individual polypeptides. DISCUSSION AND CONCLUSION: Data obtained indicate that Immunoblotting study of IgA antibody against HIV-1 polypeptides, represent an useful diagnostic complement in order to determine an active viral infection in children born to HIV-1 seropositive mothers.
Publication Types:
Keywords:
- Antibodies
- Child
- HIV Antibodies
- HIV Infections
- HIV-1
- Humans
- Immunoblotting
- Immunoglobulin A
- Immunoglobulin A, Secretory
- Immunoglobulin G
- Immunoglobulin M
- Infant
- Peptides
Other ID:
UI: 102192557
From Meeting Abstracts