Scarlatti G, Plebani A, Albert J, Marchisio P, Ferraris G, Fenyo EM, Rossi P; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 362 (abstract no. W.C.3265).
Dept. of Virology, Karolinska Institute, Stockholm, Sweden
OBJECTIVE: To study if PCR and HIV isolation reveal additional cases of pediatric HIV infection. To identify maternal factors which correlate to the risk of vertical transmission of HIV-1. METHODS: Detection of HIV-1 was attempted by polymerase chain reaction (PCR), virus isolation and antigen-assay (Ag) in 87 children born to HIV-1 infected mothers and in 47 mothers. Twenty-one mother-child pairs were followed from birth. RESULTS: HIV-1 was detected by PCR in 11 children, 10 were also virus isolation positive and 8 were Ag positive. PCR negative children (n=76) were isolation negative, antigen negative and clinically well. Seronegative children older than 18 months (n=36) were all PCR and isolation negative. Positive Ag results in the mothers correlated significantly with a higher risk of transmission; no other parameters (CDC, lymphocyte subset, PCR and isolation) showed such correlation. CONCLUSIONS: The results of PCR correlate well with other markers of vertical transmission of HIV-1 and is therefore a valid method for early diagnosis. Detectable Ag in serum of the mother correlates with an increased risk of perinatal transmission of HIV-1.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Blood Transfusion
- Child
- Disease Transmission, Vertical
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Immunologic Techniques
- Polymerase Chain Reaction
- immunology
- organization & administration
- transmission
Other ID:
UI: 102193342
From Meeting Abstracts