Krogstad DJ, Stenglein KJ, Murray DM, Eveland MR, Gluzman IY; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 205 (abstract no. S.B.477).
Washington University School of Medicine, St. Louis, Missouri, USA
OBJECTIVE: Dideoxyinosine (ddI) is a nucleoside with anti-HIV activity currently under study in a number of clinical trials. The goal of these studies was to develop a sensitive radioimmunoassay (RIA) suitable for use in pharmacokinetic studies and clinical trials because the high-performance liquid chromatography assay currently available has a limited sensitivity. METHODS: Rabbits were immunized with a ddI-bovine serum albumin conjugate with complete Freund's adjuvant and boosted monthly for 6 months using antigen with incomplete Freund's adjuvant. Serum from immunized rabbits was examined for antibody to ddI using an RIA based on 3H-ddI and goat anti-rabbit globulin. RESULTS: The RIA developed using serum from these immunized rabbits detects ddI concentrations as low as 0.3 ng per ml. Cross-reactivities with other nucleosides and purines were also low: dideoxyadenosine (0.54%), hypoxanthine (0.002%), uric acid (less than 0.005%), inosine ().0001%) and adenosine (0.001%). CONCLUSION: These results suggest that the RIA for ddI will be a useful analytical tool in current and future trials of ddI.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Anti-HIV Agents
- Cattle
- Chromatography, High Pressure Liquid
- Didanosine
- Dideoxyadenosine
- HIV
- HIV Infections
- HIV Seropositivity
- Hemagglutination Tests
- Hypoxanthine
- Inosine
- Rabbits
- Radioimmunoassay
- Uric Acid
Other ID:
UI: 102196292
From Meeting Abstracts