Nicol I, Livartowsky J, Chamaret S, Dwyer D, Montagnier L, Dormont D; Symposium on Nonhuman Primate Models for AIDS.
8th Annu Symp Nonhum Primate Models AIDS Symp Nonhum Primate Models AIDS 8th 1990 New Orleans La. 1990 Nov 28-30; 8: 134 (abstract no. 118).
Commissariat \ga l'Energie Atomique and Centre de Recherches du Service de Sant\ae des Arm\aees, Fontenay aux Roses, France
OBJECTIVE: To establish an animal model for human HIV infection. Methods: Inoculation: Eight rhesus monkeys were inoculated 3 years ago first IV and IC by 107 in vitro HIV2 ROD-infected cells, then, 1 year ago, IV reinoculated with supernatant of PBL coculture of an infected monkey which presented clinical signs of AIDS. In addition, one monkey was IV inoculated de novo with this supernatant, as a positif control of infection. Serology: HIV2 antibodies were evidenced by western blot and RIPA and neutralizing antibodies are evaluated by syncitia formation method. Viral expression: Reverse transcriptase was measured monthly in coculture with human cord blood lymphocytes. PCR: Viral integration was demonstrated in thawed PBL, using primers in gag, env and LTR sequences. Immunological status: The CD4/CD8 ratio and blood cell counts were performed. Clinical survey: Full clinical examination occurred twice a month. Results: The positive control introduced in the study became seropositive at day 70, and 3/9 animals remained seronegative except a nef reactivity in one animal. One monkey has seroconverted since the 2nd inoculation. A strong anamnestic response was observed in reinoculated animals. In coculture, 2 more animals became viremic (three are always negative). Thus, virus was transiently recovered from 6/9 monkeys, only four of them being seropositive. All animals are PCR+ and their CD4+ cells count decreased since the 2nd inoculation. The presence of lymphadenopathy is often observed and some rash skin affects chronically one animal. The new monkey introduced in this study shows a severe cachexia 7 months after inoculation. Conclusion: Rhesus monkeys are susceptible to HIV2 infection. They are seroconverting within a short period (less than 2 months) and exhibiting in most of the cases, a detectable RT activity in coculture. They also present biological and clinical signs (CD4+ cell count decrease, lymphadenopathy and weight loss). The use of a human isolate may provide the best way to approach an animal model in order to understand the pathogenesis of HIV infection. We are now determining the genetical changes between the isolate inoculated (HIV2 ROD) and isolates obtained from the inoculated animal which demonstrated clinical symptoms which may be related to HIV.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Antigens, CD4
- Antigens, CD8
- Blotting, Western
- CD4 Lymphocyte Count
- CD4-Positive T-Lymphocytes
- Genes, env
- Genes, gag
- HIV Infections
- HIV Seropositivity
- HIV-2
- Humans
- In Vitro
- Macaca mulatta
- Simian Acquired Immunodeficiency Syndrome
- genetics
- immunology
Other ID:
UI: 102197634
From Meeting Abstracts