Alvarez M, Marrero M, Valdes O, Roges G, Pera T, Bernaza J; International Conference on AIDS.
Int Conf AIDS. 1992 Jul 19-24; 8: 153 (abstract no. PuC 8005).
Institute of Tropical Medicine Pedro Kouri C. Habana, Cuba.
The aim of the present study was to compare the recently developed Polymerase Chain Reaction (PCR) technique with conventional Early Antigen Detection (EAD) and Non Radioactive Dot Blot (NRDB) hybridization for Human Cytomegalovirus (HCMV) detection in lymphocyte samples. A total of 93 heparinized blood samples, 63 from immunocompromised patients to be suspected to be HCMV infected and 30 from laboratory staff (control group). HCMV was detected in 47.3% of patients by PCR, by EAD 35.4% and by NRDB 37.5% of the patients were positive. Concordance of combined positive and negative results between different techniques was 86%, Discordant results were observed in 13 samples and 7 were positive exclusively by PCR. The high sensitivity of the PCR, either by gel detection or radioactive Dot Blot is demonstrated because HCMV was consistently detected by PCR in all specimens that were positive by at least one of the other tests.
Publication Types:
Keywords:
- Animals
- Antigens
- Antigens, Viral
- Communicable Diseases
- Cytomegalovirus
- Cytomegalovirus Infections
- Humans
- Hybridization, Genetic
- Immunocompromised Host
- Immunologic Techniques
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- diagnosis
- genetics
- immunology
- transplantation
Other ID:
UI: 102201390
From Meeting Abstracts