Gotch F, Gallimore A, Whittle H, McMichael A; Symposium on Nonhuman Primate Models for AIDS.
J Med Primatol. 1992 Sep-Oct; 21: abstract no. 14.
Institute of Molecular Medicine, Oxford, U.K.
It has been reported that some strains of HIV-2 are more closely related to SIVsm/SIVmac virus than to any known virus of human derivation. HIV-2 and SIV can cause immunodeficiency, manifested by a reduction in the number of CD4+ lymphocytes, but both viruses are thought to be less pathogenic than HIV-1. Experimental HIV-2 infections have been set up in cynomolgus monkeys and CTL have been demonstrated in these animals. CTL against proteins of immunodeficiency viruses have also been detected in HIV-1 infected humans and chimpanzees and SIV infected macaques. It has also been shown that immunisation with live recombinant vaccinia virus expressing SIV genes, or with peptide epitopes incorporated into liposomes, can induce CTL in macaques. Virus specific CTL have been shown to suppress HIV-1 or SIV replication in vitro and are thought to play an important role in maintaining good health in sero-positive individuals. Two peptide epitopes from SIV gag have been shown to be recognised by CTL in immunised or infected macaques. One of these epitopes (a.a. 41-60 in p24 gag) is recognised by CTL from infected and vaccinated rhesus macaques (restriction element MaMuA1), and by CTL from vaccinated cynomolgus macaques (restriction element unknown as yet). In rhesus macaques this epitope has been optimised to a ninemer (a.a. 47-55). This peptide is nonpolymorphic in HIV-2 and SIV, but there are three a.a. changes in HIV-1. In patients with HIV-2 we have shown a brisk CTL response to the HIV-2 gag protein. Patients with HLA B53 have been shown to respond to the same ninemer (a.a. 47-55) as the rhesus macaques. This peptide epitope was predicted by elution of endogenous peptides from purified B53 molecules. Comparisons of the structures of HLA B53, MaMuA1 and class 1 molecules from cynomolgus macaques are being undertaken.
Publication Types:
Keywords:
- Animals
- Epitopes
- Epitopes, T-Lymphocyte
- Gene Products, gag
- Genes, gag
- HIV-1
- HIV-2
- Humans
- In Vitro
- Macaca fascicularis
- Macaca mulatta
- Simian immunodeficiency virus
- T-Lymphocytes, Cytotoxic
- Vaccinia virus
- genetics
- immunology
Other ID:
UI: 102202343
From Meeting Abstracts