Remington KM, North TW, Phillips TR; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1993; 93: 445 (abstract no. T-77).
Univ. of Montana, Missoula.
The nucleoside analog, 3'-azido-3'-deoxythymidine (AZT), has been widely used for treatment of patients with the acquired immune deficiency syndrome (AIDS). Recently, human immunodeficiency virus (HIV) that is resistant to AZT has been isolated from AIDS patients that had been receiving long-term AZT therapy. The nature of these AZT-resistant variants, especially with respect to their potential for pathogenicity, is not known. It is difficult to dissect these characteristics of the mutant HIV without an animal model. We have selected, in cell culture, AZT-resistant mutants of feline immunodeficiency virus (FIV) that are very similar to the AZT-resistant clinical isolates of HIV. FIV is a lentivirus that produces a natural AIDS-like disease in cats. When plaque-purified AZT-resistant mutants of FIV were grown in the absence of AZT, they rapidly reverted to a wild-type phenotype. We have also selected and plaque-purified AZT-resistant mutants from FIV that had been derived from a genetically homogeneous molecular clone, and these also reverted rapidly. Regions of the pol gene from the molecular clone parent, the plaque-purified mutant and the plaque-purified revertants have been analyzed at the DNA sequence level. This information will be useful in understanding the mechanism of AZT-resistance.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Anti-HIV Agents
- Feline Acquired Immunodeficiency Syndrome
- Genes, pol
- HIV
- HIV Infections
- HIV Seropositivity
- Humans
- Immunodeficiency Virus, Feline
- Phenotype
- Zidovudine
- genetics
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102202568
From Meeting Abstracts