Meyaard L, Otto SA, de Jong R, Miedema F; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 32 (abstract no. WS-A16-3).
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
T cells from asymptomatic HIV-infected individuals are non-responsive to activation via the CD3/T-cell receptor complex and release of Th1 cytokines is low. Functional abnormalities of T-cells might be secondary to the disturbance of APC function due to HIV infection. Incomplete activation by affected APC might lead to anergy of T cells. Indeed, T cells from HIV-infected individuals show characteristics of anergic cells as defined in murine models, from which it is known that anergy is preferentially induced in T cells that have cytokine secretion patterns defined as Th1-like. We investigated the type of T cells that could be cloned from HIV-infected individuals. T-cells randomly cloned from HIV-infected individuals showed an increased proportion of IL-4 producing clones which produced increased levels of IL-4, compared to an HIV-negative control. In contrast, T cells cloned from the HIV-negative control produced high levels of IL-2, whereas lower IL-2 production was found in clones derived from HIV-infected individuals. The finding that preferentially Th2-like clones can be isolated after HIV-infection was further substantiated by clones derived in parallel from cryopreserved material from the same individual before infection with HIV and three years after seroconversion. Again, predominantly clones producing IL-4 were found after HIV-1 infection in contrast to the clones derived from the time point before infection. Thus, upon HIV-infection, predominantly Th2-like T-cell clones are generated which may be due to preferential anergy of Th1 cells. In addition, in vivo, Th2 cells could downregulate function of Th1 cells by secretion of cytokines as IL-10. This might contribute to the attenuation of the immune system and impaired responses to foreign antigens as observed in HIV-infected individuals.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Clone Cells
- Cytokines
- HIV
- HIV Infections
- HIV Seronegativity
- HIV Seropositivity
- Interleukin-10
- Interleukin-2
- Interleukin-4
- T-Lymphocytes
- Th1 Cells
- Th2 Cells
- immunology
Other ID:
UI: 102204002
From Meeting Abstracts