Lu YS, Ho SI, Read RW, Richardson JA, Pakes SP; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1994; 94: 485 (abstract no. T-18).
University of Texas Southwestern Medical Center, Dallas.
Similar to HIV infection in humans, cats infected with feline immunodeficiency virus (FIV) develop anemia, neutropenia and lymphopenia. These hematologic abnormalities are thought to be due to altered marrow micro-environments because of infected marrow stromal cells. Susceptibility of marrow stromal cells to FIV infection is unknown. Stromal fibroblasts from bone marrow cells isolated from three SPF cats were grown in culture medium and were exposed to FIV (Petaluma strain). Portions of the FIV-exposed stromal fibroblasts were cocultured with CRFK cells to amplify FIV infection. Culture supernatants and cells from amplified and nonamplified cultures were assayed for viral infection. Results show that cells from both cultures are positive for proviral DNA by PCR and viral antigens by IFA. However, p26 viral antigen (ELISA assay) is found only in supernatant of stromal fibroblasts cocultured with CRFK cells. Similarly, marrow stromal fibroblasts from three FIV-infected cats were prepared and treated in the same manner without exposure to FIV in vitro. Some of the stromal fibroblasts were cocultured with CRFK cells or Con A-activated peripheral blood lymphoblasts from an SPF cat. The results show that proviral DNA and viral antigens are positive in stromal fibroblasts and cocultured cells. However, p26 viral antigen is detected only in supernatant of stromal fibroblasts of individual cat cocultured with CRFK or activated lymphoblasts, but not both. Both in vitro and in vivo results indicate that feline marrow stromal fibroblasts are a target of FIV infection. FIV-infected cats may serve a good model to study hematopoietic dysfunction of HIV infection in humans.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Bone Marrow
- Bone Marrow Cells
- Cats
- Coculture Techniques
- Communicable Diseases
- Felidae
- Felis
- Fibroblasts
- HIV Infections
- Hematopoiesis
- Hematopoietic Stem Cells
- Humans
- Immunodeficiency Virus, Feline
- In Vitro
- Infection
- Polymerase Chain Reaction
- Stromal Cells
Other ID:
UI: 102207859
From Meeting Abstracts