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Evaluation of Cambridge Biotech western blot kit.

Senyonga P, Kataaha ; International Conference on AIDS.

Int Conf AIDS. 1994 Aug 7-12; 10: 245 (abstract no. PB0408).

Nakasero Blood Bank, Kampala-Uganda.

OBJECTIVES: To assess the suitability of the kit for HIV1 + 2 confirmation. MATERIALS: The kit was submitted for evaluation by the company representative, clearly labelled with: the manufacturers address, to be used for HIV1 + 2 testing, contents and quantities, storage temperature and expiry date. 26 frozen, Elisa tested and Inno Lia blotted samples were tested. METHODS: Manufacturers recommended method was used to test the samples and the 4 controls supplied by the company. RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. RESULTS ANALYSIS: In addition to P24, cambridge Biotech detected ENV. 160 probably early sero conversion in the two samples. DISCUSSION: Clear instructions and easy to carry out, specificity and sensitivity comparable to that of Innolia. The detection of ENV. 160/120 is an added advantage, indicative of early sero-conversion.

Publication Types:
  • Meeting Abstracts
Keywords:
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies
  • Genes, env
  • HIV-1
  • Oncogenes
  • Sensitivity and Specificity
  • genetics
  • methods
Other ID:
  • 94369878
UI: 102208708

From Meeting Abstracts




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