Xu Y, Murakami T, Kawase S, Uchiyama T, Hattori T; International Conference on AIDS.
Int Conf AIDS. 1994 Aug 7-12; 10: 97 (abstract no. PA0005).
Institute for Virus Research, Kyoto University, Japan.
OBJECTIVE: U937 cells were used to investigate the characters of the V3 loop binding protein (V3BP) of monocyte/macrophage. METHODS: Biotinylated V3 loop synthetic peptides from three strains of HIV-1 (IIIB, MN, ELI) were made as ligands to identify V3BP. Flow cytometric analysis was employed to quantitate V3BP. The proteins were also identified on the iodinated cells. Finally, plasma membrane was applied to the affinity columns coupled with peptides to purify V3BP. RESULTS: U937 cells gave a higher fluorescence intensity than Molt-4 cells. The molecular mass of the major radioactive bands of V3BP was found to be 32-33 kDa when radiolabeled Molt-4 and U937 cells were used. The band given by U937 was stronger than that by Molt-4 cells. A major protein(s) purified has two polypeptides (32 and 33 kDa). A size exclusion chromatography showed that the proteins migrated to a molecular mass of 130 kDa. These proteins were separated by a reverse-phase column chromatography and 32 kDa protein migrated later than 33 kDa protein. DISCUSSION AND CONCLUSIONS: V3BP of U937 cells was indicated to be a tetrameric protein, very similar to that of Molt-4 cells. The amount of V3BP on macrophage is higher than that of Molt-4 cells. These findings indicate that HIV-1 utilizes the same molecule upon infection to monocytes, but the role of V3BP on infection might be different.
Publication Types:
Keywords:
- Carrier Proteins
- Cell Membrane
- Chromatography, Gel
- HIV Antibodies
- HIV Envelope Protein gp120
- HIV Envelope Protein gp41
- HIV-1
- Molecular Weight
- Monocytes
- Peptides
- Proteins
- Receptors, CCR5
- Receptors, CXCR4
- Receptors, HIV
- U937 Cells
- immunology
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102209815
From Meeting Abstracts