Goldman BL, Ellenberger HH, Roy RR, Edgerton VR.
Abstr Soc Neurosci. 1991; 17: 649.
Dept of Kinesiology and Brain Research Institute, University of California Los Angeles 90024-1527.
The proto-oncogene c-fos is thought to encode transcriptional regulatory proteins. Northern blot analyses have demonstrated the presence of c-fos protein mRNA in skeletal muscle. In the present study, a muscle-specific selective expression of c-fos protein in muscle nuclei was induced via electrical stimulation of the sciatic nerve. Chloral hydrate-anesthetized rats were stimulated for three hours at 5-10 times the threshold needed to elicit muscle contraction. Immediately following stimulation, the rats were perfused with 2% paraformaldehyde, and the soleus and tibialis anterior (TA) muscles were removed and quick frozen in isopentane cooled in liquid nitrogen. Control rats were anesthetized and the sciatic nerve was isolated but not stimulated. Immunohistochemical detection using polyclonal antiserum to the n-terminal region of the c-fos protein showed extensive labeling of nuclei in the stimulated, but little or no labeling of nuclei in the non-stimulated soleus. There also appeared to be a greater number of immunoreactive nuclei in the stimulated than in the non-stimulated TA. The proportion of nuclei labeled in the stimulated soleus far exceeded that observed in the stimulated TA. These data are consistent with the hypothesis that myonuclear expression of immediate-early genes such as c-fos can play a role in muscle adaptation.
Publication Types:
Keywords:
- Animals
- Cell Nucleus
- Cesium
- Chromosomes
- DNA-Binding Proteins
- Electric Stimulation
- Gene Expression
- Gene Expression Regulation
- Genes, Immediate-Early
- Genes, fos
- Hindlimb
- Lower Extremity
- Muridae
- Muscle Fibers
- Muscle, Skeletal
- Muscles
- Proto-Oncogenes
- Rats
- Rats, Sprague-Dawley
- Sciatic Nerve
- genetics
- NASA Discipline Musculoskeletal
- NASA Discipline Number 00-00
- NASA Discipline Number 26-10
- NASA Program Flight
- NASA Program Space Physiology and Countermeasures
- Non-NASA Center
Other ID:
UI: 102212516
From Meeting Abstracts