Zapiola I, Bouzas MB, Lopez E, Fallo A, Sordelli N, Cattaneo A, Wainstein C, Muchinik G; National Conference on Human Retroviruses and Related Infections.
Program Abstr Second Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 2nd 1995 Wash DC. 1995 Jan 29-Feb 2; 121.
Hospital de Ninos de Buenos Aires "Ricardo Gutierrez" and Programa SIDA, MCBA; Buenos Aires, Argentina.
We evaluate the test of p24 antigen, as a diagnostic tool, in low budget locations, as a follow up test in pediatric patients born to HIV-positive mothers. One hundred and seven samples were collected from 92 patients. Abbott HIV-1 Ag. monoclonal and Abbott HIV-1/2 Ab assay was used. Serum disruption of immune complexes (IC) were dissociated (D) using an in home ICD assay. All positive samples were neutralized (Abbott Lab.). In children greater than 18 months of age the p24 assay showed a correlation between the presence or absence of ab. (52 out of 53 [93%] samples). In children younger than 18 months of age, only 1 (ELISA+, WB-) out of 37 A. seronegative samples show positive the Ag. test. In 11 indet. Ab. assays, the p24 test resulted +; in 5 patients the Ag-p24 define their AIDS status, four of them, before 9 months of age. Although preliminary, the p24 Ag test seems very useful as the only additional surrogate marker for pediatric studies in undeveloped countries.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigen-Antibody Complex
- Biological Assay
- Biomedical Research
- Child
- Enzyme-Linked Immunosorbent Assay
- HIV Infections
- Humans
- Research Design
- immunology
- organization & administration
Other ID:
UI: 102213341
From Meeting Abstracts