Ndimbie OK, Perry B, Deriggi C, Guiliani R; National Conference on Human Retroviruses and Related Infections.

Program Abstr Second Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 2nd 1995 Wash DC. 1995 Jan 29-Feb 2; 123.

Central Blood Bank and University of Pittsburgh, Pittsburgh, PA.

Patients with indeterminate human immunodeficiency virus 1 (HIV-1) immunoblots are difficult to counsel. Clinical options include retesting by enzyme immunoassay (EIA) and Western blot (WB) in three to six months or assaying by PCR or immunofluorescence. We evaluated the Murex Single Use Diagnostic System (SUDS R, Murex Corporation, Norcross, GA), a rapid microfiltration EIA for the detection of antibodies to HIV-1, to determine whether or not it could resolve the WB indeterminates. The test system detects antibodies to p24 and gp41. 57 serum samples which had previously been tested by EIA (Abbott, N. Chicago, IL) and WB (Epitope, Inc. Beaverton, OR) were reanalyzed by SUDS. CDC criteria were used for interpretation of the WB. Of the 57 samples, 12 were EIA negative, and 45 were EIA positive. 21, 19, and 17 were WB negative (N), indeterminate (I), and positive (P), respectively. EIA negative samples were WB N except one which was WB I and SUDS negative. The sensitivity and specificity of SUDS for WB N and P samples was 100%. 14 of 19 (74%) of WB I samples were SUDS negative. Five WB I samples were SUDS positive (four persons). Follow-up data was available on three of the four. One tested PCR positive, another tested WB P eight months later, one was still WB I six months later, and one was lost-to-follow-up. The person who remained WB I was consistently positive for antibody to envelope proteins. A review of the reactive bands of the other WB I showed a variety including nonviral, and multiple weak positive and positive envelope, core, and polymerase bands. The SUDS assay may be used in conjunction with WB bands to resolve WB I. Negative SUDS are likely negative. "False" positive SUDS is seen in an occasional WB I with envelope bands. Caution should be exercised in forecasting results of other assays.

Publication Types:

• Meeting Abstracts

Keywords:

• Blotting, Western
• Chicago
• Fluorescent Antibody Technique
• HIV-1
• Humans
• Immunoenzyme Techniques
• Sensitivity and Specificity

Other ID:

• 95920402

UI: 102213351

From Meeting Abstracts