Lee CH, Lu JJ, Atzori C, Orlando G, Cargnel A, Bartlett MS, Smith JW; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1996 May 19-23; 75 (abstract no. F-9).
Indiana University School of Medicine, Indianapolis, IN.
Using the P. carinii typing method that we developed, we have examined specimens from 167 patients, including 122 from Indiana University Medical Center (IUMC), 26 from Italy, 10 from Denmark, 6 from Thailand, and 3 from Africa. Among the 122 specimens from IUMC, 87 were from AIDS patients and 35 from patients with other diseases. Specimens from other countries were all from AIDS patients. DNAs isolated from these specimens were used as templates for PCR. The region containing both internal transcribed spacers (ITS1 and ITS2) of rRNA genes was amplified. The PCR products were sequenced or probed with type-specific probes to determine P. carinii types. Ninety-nine (60 percent) of the 167 patients were found to be infected by one type of P. carinii, and 68 (40 percent) patients were infected by more than one type of P. carinii. Among the IUMC AIDS patients, type III P. carinii was found to be most prevalent (38 percent), followed by type II (26 percent) and then types I (18 percent) and IV (18 percent). The order of prevalence of P. carinii types in the non-AIDS IUMC patients was type III (42 percent) followed by types I (23 percent), II (19 percent), and IV (16 percent). In Italy, type III was found to be most prevalent (37 percent), followed by types IV (33 percent), I (27 percent), and II (3 percent). Since only a few specimens from Denmark, Thailand, and Africa were examined, the true prevalence of P. carinii types in these areas remains to be determined. The results of this study indicate a difference in the prevalence of various P. carinii types in different geographical locations.
Publication Types:
Keywords:
- Africa
- Base Sequence
- Denmark
- Epidemiology, Molecular
- Genes, rRNA
- Humans
- Indiana
- Italy
- Pneumocystis Infections
- Pneumonia, Pneumocystis
- Polymerase Chain Reaction
- Thailand
- genetics
Other ID:
UI: 102222975
From Meeting Abstracts