Amjad M, Pomerantz RJ, Bagasra O; Conference on Retroviruses and Opportunistic Infections.
Program Abstr 4th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 4th 1997 Wash DC. 1997 Jan 22-26; 4th: 82 (abstract no. 100).
Dorrance H. Hamilton Laboratories, Center for Human Virology, Philadelphia, PA.
There has been controversy regarding identity of the HIV-1 suppressive factor to be either beta-chemokines or the Levy factor as reported by Cocchi, et al. and Levy, et al. 1986. This report is an attempt to resolve this issue. We generated anti-HIV-1 factors from a well-characterized long-term non-progressor (LTNP). The anti-HIV-1 effects of the supernatants from the unfractionated PBMC, CD8+ and CD4+ enriched subsets from this individual were tested on several primary HIV-1 isolates (301660, 93IN101, and CMU08 representing subtypes B,C and E respectively) and various laboratory strains (HIV-1 NL4-3 and 89.6). 10% final concentration of the suppressive factors showed inhibition of HIV-1 replication up to 76%, as determined by reverse transcriptase (RT) assay over the period of 12 days. A cocktail of RANTES, MIP-1alpha and MIP-1beta (at 100ng/ml) were utilized to determine the degree of HIV-1 inhibition of the above HIV-1 primary isolates and laboratory strains. The degree of inhibition of HIV-1 was significantly less (less than 40%) than reported previously by Cocchi, et al. We further evaluated the role of beta-chemokines in the supernatants collected from the PBMCs and subsets from the LTNP studied. For this purpose, we utilized specific polyclonal goat IgG neutralizing antibodies (NAb) against RANTES, MIP-1alpha and MIP-beta. For all the primary HIV-1 isolates, neutralization of the suppressive factor from the unfractionated PBMC ranged from 32% to 56% on any given day over the time period of 10 days. No neutralization of CD8+ T cell anti-factors (CAF) and CD4+ suppressive factor was observed for the primary HIV-1 isolate 301660. For HIV-1 isolate 93IN101 neutralization of CAF was not observed, however 100% neutralization of CD4+ specific factor was achieved. For HIV-1 NL4-3 neutralization of the LTNP suppressive factor was only 13.8%. Our results suggest that anti-HIV-1 effects exerted by CAF may be different than the chemokine/chemokine receptor mediated HIV-1-inhibition reported in several recent articles.
Publication Types:
Keywords:
- Antigens, CD4
- Antigens, CD8
- Chemokine CCL5
- Chemokines
- Chemokines, CC
- HIV-1
- Macrophage Inflammatory Proteins
- RNA-Directed DNA Polymerase
- immunology
Other ID:
UI: 102223282
From Meeting Abstracts