Utsunomiya T, Kobayashi M, Pollard RB, Ito M, Suzuki F; International Conference on AIDS.
Int Conf AIDS. 1998; 12: 29 (abstract no. 11234).
University of Texas Medical Branch, Galveston 77555-0835, USA.
OBJECTIVE: It has been reported that type 2 T cell responses (T2 responses) play an important role in the pathogeneses of Candida albicans (C. albicans) infection in immunocompromized hosts. A predominance of T2 responses has been shown in MAIDS mice. As the modulatory activity of glycyrrhizin (GR) on the differentiation of T cells into T2 cells was demonstrated, the present study investigated the anti-fungal effects of GR on C. albicans infection in MAIDS mice. METHODS: GR was kindly supplied by Minophagen Pharmaceutical Co., Tokyo, Japan. C57BL/6 mice were inoculated i.p. with 0.1 ml of culture fluids of SC-1 cells persistently infected with LP-BM5 MuLV. Eleven weeks atfer the viral infection, these mice were exposed to 1 x 10(5) cells/mouse of C. albicans, and treated with GR every other day beginning 3 days before fungal infection for a total of 10 injections. For the production of cytokines, splenic mononuclear cell (SMNC) from mice 11 weels after the viral infection were stimulated with 10 micrograms/ml of anti-CD3 mAb for various hrs. RESULTS: The susceptibility of MAIDS mice to the fungal infection was 100 times higher than that of normal mice. Also, normal mice treated with a mixture of type 2 cytokines (IL-4 and IL-10) were susceptible to C. albicans infection at the same levels observed in MAIDS mice. In MAIDS mice treated with GR, the susceptibility to infection from these pathogens was recovered to levels observed in normal mice. After stimulation with anti-CD3 mAb, SMNC from MAIDS mice produced type 2 cytokines into their culture fluids. However, these cytokines produced were not demonstrated in cultures of SMNC from MAIDS mice treated with GR. CONCLUSIONS: An opportunistic infection of C. albicans in MAIDS mice was controlled by GR. The increased susceptibility of MAIDS mice to fungal infection was influenced by T2 responses induced by LP-BM5 MuLV, and GR regulated the development of MAIDS-associated T2 responses. These results suggest that GR may have an anti-fungal activity on C. albicans infection in MAIDS mice.
Publication Types:
Keywords:
- Animals
- Antibodies, Monoclonal
- Candida albicans
- Candidiasis
- Cytokines
- Glycyrrhizic Acid
- Interleukin-10
- Interleukin-4
- Japan
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Opportunistic Infections
- T-Lymphocytes
- Tokyo
- visilizumab
Other ID:
UI: 102226788
From Meeting Abstracts