Schoer D, Mitteco V, Gottfried T, Kanar M; International Conference on AIDS.

Int Conf AIDS. 1998; 12: 765 (abstract no. 41114).

Calypte Biomedical, Berkeley, CA 94710, USA.

OBJECTIVE: To evaluate the performance of a modified Calypte HIV-1 urine EIA test kit. METHODS: The performance of a modified Calypte HIV-1 urine EIA was evaluated with a total of 2027 urine specimens and ten dilution panels. Urine specimens used in this study were collected both retrospectively and prospectively. The EIA protocol was as described in the package insert with minor modifications. EIA reactive specimens were repeated in duplicate. Repeatedly reactive specimens were confirmed by urine western blot. In the retrospective study conducted at Calypte, 1027 urine specimens were assayed. These specimens represented the following categories: 1) HIV-1 seropositive, 2) HIV-1 seronegative individuals considered at high risk for HIV infection, 3) individuals considered at low risk for HIV infection and 4) ten dilution panels. In the prospective study, 1000 specimens from individuals considered at low risk for HIV infection were assayed by an independent laboratory. RESULTS: The data are summarized in the table below. In this study, the sensitivity and specificity of the modified Calypte HIV-1 urine EIA test kit were 100% and 99.5% respectively. The false positive rate was lower among samples from the low risk population than the high risk population. The combined specificity for both high and low risk groups was 99.4%. Performance of the modified kit with 9 of 10 HIV-1 urine dilution panels was equivalent to the current kit. With one panel, the modified kit was more sensitive, giving a positive signal one dilution earlier. TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: The modified Calypte HIV-1 urine EIA has excellent sensitivity and specificity. The availability of a urine based test system provides a reliable, cost effective tool for diagnosing HIV-1 infection and preventing the spread of AIDS.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Animals
• Behavior
• Blotting, Western
• Equine Infectious Anemia
• HIV Infections
• HIV-1
• Immunoenzyme Techniques
• Population
• Prospective Studies
• Prostitution
• Retrospective Studies
• Sensitivity and Specificity
• blood
• urine

Other ID:

• 98402732

UI: 102230764

From Meeting Abstracts