Hofgartner WT, Plorde JJ, Fritsche TR; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1997 May 4-8; 97: 581 (abstract no. V43).
University of Washington, Seattle, WA.
Toxoplasma gondii is an important pathogen in immunocompromised patients and a cause of birth defects in congenitally infected infants. From 5% to 25% of the adult population in the USA is seropositive for Toxoplasma antibodies. Acceptable sensitivity and specificity have been reported for the measurement of IgG antibodies, but the detection of IgM antibodies is more problematic because of clinical concerns. METHODS: We performed a comparative evaluation of the following immunoassays: Behring Diagnostics OPUS Toxo G/Toxo M; Abbott Diagnostics IMX Toxo-IgG 2.0/Toxo-IgM; Sanofi Diagnostics Pasteur Platelia Toxo IgG/Toxo IgM; bioMerieux Vitek VIDAS Toxo IgG/IgM. Patient specimens referred to our laboratories for T. gondii antibody testing were included. RESULTS: 676 specimens were tested for IgG and 718 specimens for IgM antibodies. Of the specimens tested for IgG, 66% were nonreactive and 26% were reactive by all 4 methods. Of the specimens tested for IgM, 87% were nonreactive and 3% were reactive by all 4 methods. Overall agreement of the 4 methods was 92% for IgG and 90% for IgM. Of the 56 discrepant IgG specimens and the 73 discrepant IgM specimens each system was discrepant with all 3 others with the following, respective frequencies: OPUS 20% and 51%; IMX 29% and 19%; Platelia 14% and 8%; VIDAS 5% and 7%. CONCLUSION: Overall agreement between the 4 different methods is good for both IgG and IgM. Further evaluation of discrepant specimens may provide additional information in explaining particular differences.
Publication Types:
Keywords:
- Adult
- Animals
- Antibodies, Protozoan
- Evaluation Studies
- Humans
- Immunoassay
- Indicators and Reagents
- Infant
- Reagent Kits, Diagnostic
- Sensitivity and Specificity
- Toxoplasma
- methods
Other ID:
UI: 102235562
From Meeting Abstracts