Colgrove R, Pitt J, Chung PH, Welles S, Japour A; Conference on Retroviruses and Opportunistic Infections.
Program Abstr 5th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 5th 1998 Chic Ill. 1998 Feb 1-5; 5th: 129 (abstract no. 265).
Harvard Medical School, Boston, MA.
HIV-1 Reverse Transcriptase codons 10 through 250 were sequenced from 24 pairs of zidovudine-exposed women and their HIV-infected infants enrolled in the Women and Infants Transmission Study (WITS). HIV sequences were analyzed by automated fluorescent dye-primer cycle sequencing for the transmission of sequence variants from mother to infant. For 17 of 24 (71%) of these pairs, maternal and infant sequences were identical to one another and lacking known zidovudine resistance mutations. 7 (29%) maternal sequences contained mutations at RT codons 70, 210, 215 and 219, all known to be associated with zidovudine resistance. When the maternal HIV isolate showed a chromatographically pure mutant species, the infant sequence was identical (n=4). When the maternal sequence showed the presence of a sequence mixture at codon 70 or 219 (n=2), the infant's virus showed only wild-type sequence even when the zidovudine-resistant mutant was quantitatively dominant in the mother. The single maternal HIV isolate showing mixed sequence at codon positions 210 and 215 transmitted a chromatographically unmixed mutant to the infant at both positions. When maternal mixtures were present at other sites not known to be associated with zidovudine resistance, only the dominant species appeared in the infant. These data suggest that infection in the infant is established by a very small number of maternally-derived virions and may indicate a difference in fitness for transmission among different resistance mutations.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Base Sequence
- Codon
- Disease Transmission, Vertical
- Female
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Infant
- Mutation
- Zidovudine
- genetics
- reverse transcriptase, Human immunodeficiency virus 1
- transmission
Other ID:
UI: 102235845
From Meeting Abstracts