Livrozet JM, Jeanblanc F, Brunel F, Makhloufi D, Saint-Marc T, Touraine JL, Aymard M, Tardy JC; Conference on Retroviruses and Opportunistic Infections.
Program Abstr 5th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 5th 1998 Chic Ill. 1998 Feb 1-5; 5th: 142 (abstract no. 336).
Hopital Edouard Herriot, Lyon, France.
Objective: To determine the prevalence of T215 Y/F, L74V, T69D, and M184 V mutations among naive patients. Studied population and methods: A nested PCR - based differential probe hybridation was used for the determination of mutant and wild-type species in plasma virus RNA. It was applied to the study of 70 asymptomatic, naive patients. The mean HIV RNA (Amplicor Monitor Roche) was 4.4 log 10. Results: The sensitivity of the assay enabled us to detect 250 copies/ml. In a mixed population containing both resistant and sensitive species, this technique can identify a minor resistant population with a threshold of 10 %. Applied to the study of naive patients, the assays showed the presence of the mutation 215 in only one patient (1.4%). The other mutations (74, 69, 184) were never found. Conclusion: Among naive patients, mutations confirming resistance to NRTIs are rare in plasma virus RNA. The determination of genotype susceptibility does not seem necessary at the present time, before the initiation of the first treatment, although it is important to be carried out in patients who have already been treated with NRTIs.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Biomedical Research
- Genotype
- HIV Infections
- Humans
- Prevalence
- RNA, Viral
- genetics
Other ID:
UI: 102235916
From Meeting Abstracts