Garneau M, Bolger GT, Bousquet C, Kibler P, Cordingley MG; International Conference on Antiviral Research.
Antiviral Res. 1998 Mar; 37: A87 (abstract no. 170).
Bio-Mega Research Division, Boehringer Ingelheim Ltd., Quebec, Canada.
Cytomegalovirus associated retinitis is a major cause of vision loss in the AIDS patient. A safer and more effective antiviral treatment that would prevent development of retinitis represents an ongoing effort in research. While histopathology or viral titration are the preferred methods to evaluate retinitis in murine cytomegalovirus (MCMV) models, we investigated the use of electroretinography (ERG) as a noninvasive technique to measure the functional activity of the retina following MCMV anterior chamber inoculation. While under anesthesia, a volume of 1.9 microliter containing either control salivary gland homogenate or 400 pfu of salivary gland passed, Smith Strain MCMV was injected in the anterior chamber of the right eye of 6 to 8 week old SCID mice. At various times post inoculation, scotopic ERG recordings were obtained from the injected eyes. The wave amplitudes were compared. In mice inoculated with control salivary gland homogenate, no change in A or B wave amplitude was noted, up to day 21 post inoculation. Both the A-wave and the B wave amplitude recorded from infected eyes were significantly reduced (p less than 0.05,; ANOVA SNK multiple comparison) as of day 10 post inoculation. When antiviral therapy using HPMPC (5 mg/kg/day s.c. once daily for 5 consecutive days) was started 3 hr post inoculation, no significant changes were observed in the different ERG parameters up to day 21 post inoculation. Viral titration in these eyes revealed higher viral titer in the injected eyes of non treated animals compared to HPMPC treated animals. Histopathological examination of the eyes revealed a good correlation between the ERG B wave amplitude and the extent of retinal disease. These results suggest that ERG recording represent a valuable noninvasive technique to measure retinal disease following MCMV anterior chamber inoculation in SCID mouse.
Publication Types:
Keywords:
- Animals
- Anterior Chamber
- Antiviral Agents
- Biomedical Research
- Cytomegalovirus
- Cytomegalovirus Infections
- Cytosine
- Electroretinography
- Eye
- Eye Infections, Viral
- Humans
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Mutant Strains
- Mice, SCID
- Muromegalovirus
- Phosphonic Acids
- Research
- Retina
- Retinitis
- cidofovir
- utilization
Other ID:
UI: 102236650
From Meeting Abstracts