Hu J, Pope M, Brown C, O'Doherty U, Miller CJ; Keystone Symposia on Molecular and Cellular Biology: 1998 HIV Pathogenesis and Treatment.

Keyst Symp Mol Cell Biol Keyst Symp Mol Cell Biol. 1998 Mar 13-19; 91 (abstract no. 4042).

University of California, Davis, CA.

Our previous studies have shown that SIV-infected cells within the stratified squamous epithelium of the vagina have a dendritic morphology. However the type of cell infected was not conclusively determined. Combined in situ hybridization and immunostaining was used to immunophenotype the SIV-infected cells in the lower reproductive tract and genital lymph nodes of the female rhesus monkey. We now show that SIV-infected cells were most common in iliac lymph node and the majority of infected cells in the lymph node were T lymphocytes. SIV-infected macrophages, Langerhans cells (LC) and dendritic cells were also found in the lymph node. While most of the infected cells in the vagina were T cells, a significant proportion (approximately 40%) of the SIV-infected cells in cytospin preparations from vagina and cervix were LC. This study provides the first direct demonstration that LC and dendritic cells in the genital tract and lymph nodes are infected with SIV in vivo. Thus, dendritic cells in general, and LC in particular, are important for HIV/SIV replication in vivo.

Publication Types:

• Meeting Abstracts

Keywords:

• Animals
• Cervix Uteri
• Dendritic Cells
• Female
• HIV
• HIV Infections
• Immunophenotyping
• Langerhans Cells
• Lymph Nodes
• Macaca mulatta
• Macrophages
• Simian immunodeficiency virus
• T-Lymphocytes
• Vagina

Other ID:

• 98930465

UI: 102236893

From Meeting Abstracts